Isolation, characterization and identification of nitrogen fixing bacteria with organic fertilizer applications in paddy soil

Abstract The high organic matter content allows soil microbial activity to recycle matter, which is essential for life, such as the nitrogen cycle. process of biological fixation by microorganisms crucial entry into This study aims isolate and characterize nitrogen-fixing bacteria in paddy using fertilization applications. was conducted Salassae Village, Bulukumba, South Sulawesi, Laboratory Microbiology, Soil Science Department, Hasanuddi University. research method a descriptive morphological Characterization, biochemical tests, molecular identification. succeeded isolating ten bacterial isolates from wetland samples fertilizers. Morphological characterization results showed quite different terms color, size, shape, level. highest ability test obtained isolates. L1.P, where 0.26%. Biochemical Characterization VITEK®. 02 shows that almost all tests show positive reaction. identification Nitrogen-fixing species 16S rRNA gene sequencing L1.P identified with 99% similarity Bacillus subtilis subsp. subtillis str. 168 .